Performance comparison between solid phase extraction and magnetic carbon nanotubes facilitated dispersive-micro solid phase extractions (Mag-CNTs/d-µSPE) of a cyanide metabolite in biological samples using GC–MS

Abstract Dispersive-micro solid phase extraction (d-µSPE) has gained increasing attention due to its convenience, effectiveness, and flexibility for sorbent selection. Among a various selection of materials, magnetic carbon nanotubes (Mag-CNTs) is promising d-µSPE with excellent separation efficiency in addition high surface area adsorption capability. In this work, two different surface-modified Mag-CNTs, Mag-CNTs-COOH Mag-CNTs-SO 3 H, were developed facilitate (Mag-CNTs/d-µSPE). The cyanide metabolite, 2-aminothiazoline-4-carboxylic acid (ATCA), was selected evaluate their performance using gas chromatography–mass spectrometry (GC–MS) analysis. enabled one-step derivatization/desorption approach the workflow; therefore, better overall achieved. Compared H/d-µSPE SPE workflow, desorption/derivatization improved reduced solvent consumption waste production. Both Mag-CNTs/d-µSPE workflows validated according ANSI/ASB 036 guidelines showed analytical performances. limit detection (LOD) quantitation (LOQ) ATCA synthetic urine 5 10 ng/mL, respectively, that bovine blood achieved at 60 ng/mL. method’s LOD LOQ also determined 1 25 ng/mL samples. methods demonstrated great potential extract polar ionic metabolites from biological matrices. processes described work can provide an easier-to-adopt procedure routine forensic testing stable biomarker poisoning cases, particularly those cases where window passed.